In India, healthcare startup incubators have become important facilitators of innovation, entrepreneurship, and technology-based change in the national healthcare environment. The last ten years have seen the country experience a high development of digital health solutions, the development of medical devices, telemedicine platforms, AI-based diagnostics, biotechnology projects, and preventive healthcare technologies. In this regard, incubators assisted by government agencies, universities, and private entities have moved beyond their conventional functions of providing workspace and seed funding and now offer mentorship services, regulatory guidance, clinical pathways, intellectual property support, prototyping services, investment contacts, and market access services. Nonetheless, with this growth, the performance of healthcare incubators has been uneven, with a significant number of them experiencing challenges in area-specific support, clinical relationship, long-term funding, and formalized commercialization assistance. The proposed study was a cross-sectional mixed-methods study design, which was intended to assess the strengths, weaknesses, and gaps in the healthcare healthcare startup incubators in India. Structured questionnaires that were sent to incubator managers, startup founders, mentors, and incubation teams helped to gather quantitative data, and semi-structured interviews with the key stakeholders such as program directors, regulatory consultants, healthcare innovators, and industry experts were used to obtain qualitative data. Secondary data was also analyzed based on annual incubator reports, government policy reports and public startup performance datasets which were used to substantiate findings. The findings proved that mentorship assistance, networking facilities, and initial technical advice were viewed as the most potent aspects of incubation. Conversely, regulatory facilitation, access of clinical validation facilities, medical-grade prototyping infrastructure, and post-incubation commercialization pathways were all debilitative gaps that were always cited. Much variation was found among the models of incubators with academic based incubators generally doing a better job in the area of clinical networking and government backed incubators providing a better institutional support at the cost of slower operational flexibility.